Lack of inducibility of antiquitin (ALDH7A1) in cultured human embryonic kidney (HEK293) cell under osmotic or oxidative stress
Refereed conference paper presented and published in conference proceedings


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AbstractThe deduced amino acid sequences of antiquitins (ALDH7) from plants and animals exhibit about 60% homology, indicating that the protein might play an important evolutionarily conserved role. The antiquitin mRNA level is up-regulated in the wilting shoot of Pisum sativum, and in the leaf and root of Brassica napes and Hordeum. vulgare under osmotic stress. Antiquitin is thus believed to play a role in some osmotic adaptive pathways in the cell, although its specific action remains unclear. Antiquitin is found to be constitutively expressed in human tissues, especially in the kidney. The antiquitin mRNA level is also high in the cochlea, where osmotic balance must be maintained for proper functioning. However, whether this enzyme is inducible under conditions of osmotic stress in human cells has not been systematically studied. In this study, a human embryonic kidney (HEK293) cell line was used to study the inducibility of antiquitin under various conditions of stress. The cells were subjected to different regimens of hyperosmotic stress for 6 h with the inclusion of salt (NaCl), sugars (sucrose, glucose and fructose), or sugar alcohols (sorbitol and mannitol) in the culture medium. The cells were also subjected to chemical hypoxia, heat shock, t-butylhydroperoxide treatment, hydrogen peroxide treatment and forskolin challenge. The antiquitin mRNA levels were analyzed by reverse transcription polymerase chain reactions using gene specific primers. Under all these conditions, no increase in the antiquitin mRNA level was registered. In some cases, a slight down-regulation was noted. Thus, it appears that antiquitin in this human cell line did not respond to the applied stress conditions. This is in contrast to the situation in plants, where antiquitin has been demonstrated previously to be inducible under stress.
All Author(s) ListWong WY, Cheng CHK, Fong WP
Name of Conference12th Meeting on Enzymology and Molecular Biology of Carbonyl Metabolism
Start Date of Conference06/07/2004
End Date of Conference11/07/2004
Place of ConferenceBurlington
Country/Region of ConferenceUnited States of America
Detailed descriptioned. by Henry Weiner, Bryce Plapp, Ronald Lindahl, and Edmund Maser.
Year2006
Month1
Day1
Volume Number12
PublisherPURDUE UNIV PRESS
Pages96 - 103
ISBN1-55753-384-9
LanguagesEnglish-United Kingdom
Keywordsaldehyde dehydrogenase; antiquitin; inducibility; osmoregulation
Web of Science Subject CategoriesBiochemistry & Molecular Biology

Last updated on 2021-27-01 at 23:27