Celecoxib induces dose dependent growth inhibition in nasopharyngeal carcinoma cell lines independent of cyclooxygenase-2 expression
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摘要Celecoxib is a cyclooxygenase-2 (COX-2) selective non-steroidal anti-inflammatory drug (NSAID) which has been shown to be capable of inhibiting the growth of various cancer cell lines. However, studies of its effect on the growth of nasopharyngeal carcinoma (NPC) cells are scarce. In this study, we investigated the effect of celecoxib on cell growth using three NPC cell lines: HK-1, Hone-1 and CNE-2. Our results showed that while all 3 cell lines expressed the COX-2 mRNA as determined by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis showed that only HK-1 expressed the COX-2 protein. Using MTT assay, celecoxib was found to inhibit growth in all 3 cell lines in a dose dependent manner. The lC(50) of celecoxib were 41.04 +/- 1.22, 49.68 +/- 1.12 and 51.74 +/- 3.89 mu M for CNE-2, Hone-1 and HK-1 cells, respectively. This growth inhibitory effect was found to be independent of the cell line's COX-2 protein expression level of the cells lines. In HK-1 cells, the expression of the cell cycle regulatory protein, cyclin D1, was down regulated by incubation with 80 mu M celecoxib for 24 hrs. (c) 2005 Elsevier SAS. All rights reserved.
著者Chan CML, Ma BBY, Wong SC, Chan ATC
會議名稱1st Annual Conference of the Organization-for-Oncology-and-Transitional-Research
會議開始日15.10.2004
會議完結日16.10.2004
會議地點Hong Kong
期刊名稱Biomedicine and Pharmacotherapy
詳細描述suppl2.
出版年份2005
月份10
日期1
卷號59
出版社ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
頁次S268 - S271
國際標準期刊號0753-3322
電子國際標準期刊號1950-6007
語言英式英語
關鍵詞celecoxib; cyclooxygenase-2; nasopharyngeal carcinoma
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Research & Experimental Medicine

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