Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11
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Abstractinfection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with all scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01 (c) 2005 Elsevier Inc. All rights reserved.
All Author(s) ListCheng M, Chan CWL, Cheung RCF, Bikkavilli RK, Zhao Q, Au SWN, Chan PKS, Lee SST, Cheng G, Ho WKK, Cheung WT
Journal nameBiochemical and Biophysical Research Communications
Volume Number338
Issue Number3
Pages1654 - 1660
LanguagesEnglish-United Kingdom
Keywordsantibody interleukin 11; nucleocapsid protein; phage-display; SARS-coronavirus; scFv
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

Last updated on 2020-25-09 at 13:42