The role of Raf-1 in radiation resistance of human hepatocellular carcinoma Hep G2 cells
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AbstractDevelopment of radiation resistance is one of the major reasons that cancer cells do not respond to radiotherapy and the mechanism for resistance is still not clear. Two sublines of human hepatocellular carcinoma Hep G2 cells were established from cells that survived two different irradiation regimes, 2 Gy for 10 days or 10 Gy for 2 days, respectively. Using MTT assay, the radiation conditioned cells were found to be more resistant to gamma-irradiation and have a greater extent of potentially lethal damage repair (PLDR) for radiation than the parent cells. By Western blot analysis, the radiation-conditioned cells were found to over-express Raf-1 which is known to regulate the radiation-resistance of cells. Inhibition of Raf-1 expression by antisense oligonucleotides increased the radiation sensitivity of the radiation-conditioned cells while inhibitors of Ras (L744,832), PI3K (LY294002) and p38 (SB203580) had no effect. Moreover, antisense Raf-1 oligonucleotides also decreased the radiation induced PLDR capacity of the radiation conditioned cells. It is therefore suggested that Raf-1 may induce radiation resistance through an increase in radiation induced PLDR capacity in Hep G2 cells.
All Author(s) ListTang WY, Chau SPY, Tsang WP, Kong SK, Kwok TT
Journal nameOncology Reports
Volume Number12
Issue Number6
Pages1349 - 1354
LanguagesEnglish-United Kingdom
Keywordshepatocellular carcinoma; radiation resistance; Raf-1
Web of Science Subject CategoriesOncology; ONCOLOGY

Last updated on 2021-25-02 at 01:49