Quantitative detection of methylated SOCS-1, a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection
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AbstractAlthough methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72degreesC extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.
All Author(s) ListChan MWY, Chu ESH, To KF, Leung WK
Journal nameBiotechnology Letters
Year2004
Month8
Day1
Volume Number26
Issue Number16
PublisherKLUWER ACADEMIC PUBL
Pages1289 - 1293
ISSN0141-5492
LanguagesEnglish-United Kingdom
Keywordsgastric cancer; primer dimers; promoter hypermethylation; real time MSP; SOCS-1; SYBR green
Web of Science Subject CategoriesBiotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Last updated on 2020-03-06 at 00:16