Effects of food deprivation on expression of growth hormone receptor and proximate composition in liver of black seabream Acanthopagrus schlegeli
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AbstractThe effects of food deprivation on the hepatic level growth hormone receptor (GHR) were investigated in black seabream (Acanthopagrus schlegeli) both at the protein level (by radioreceptor assay) and at the mRNA level (by ribonuclease protection assay). Serum levels of growth hormone (GH) and triiodothyronine (T-3) were also measured. Condition factor and hepatic proximate composition of the fish were also assessed. Significant decrease in hepatic GHR binding was recorded as early as on day 2 of starvation. On day 30 this decrease was even more pronounced, with the level in the starved fish reaching less than 20% the fed control level. A concomitant decrease in the hepatic GHR mRNA content was also noted during this period, with a progressive decrease from day 2 to day 30 of starvation. The extent of decrease in the mRNA content was less pronounced than the decrease in receptor binding, with the hepatic GHR mRNA content in the day 30 starved fish representing approximately 30% of the level in the fed control. In large contrast, serum GH level increased progressively during starvation. After 30 days of starvation, serum GH levels in the starved fish were more than three times the concentration found in the fed control. Serum T-3 levels, on the other hand, decreased during starvation, with the difference reaching significance on day 15 and day 30. After 30 days of starvation, serum T3 levels in the starved fish were only approximately 40% the concentration found in the fed control. The hepatic lipid content exhibited an increasing trend during starvation. On day 30 the hepatic lipid content of the starved fish had doubled the level found in the fed control. However, the hepatic protein content did not exhibit much change during starvation. There was also a minor decrease in the moisture content of the liver during starvation, but the condition factor of the fish as a whole registered a gradual decrease during the course of food deprivation. (C) 2004 Elsevier Inc. All rights reserved.
All Author(s) ListDeng L, Zhang WM, Lin HR, Cheng CHK
Journal nameComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
Year2004
Month4
Day1
Volume Number137
Issue Number4
PublisherELSEVIER SCIENCE INC
Pages421 - 432
ISSN1096-4959
LanguagesEnglish-United Kingdom
KeywordsAcanthopagrus schlegeli; black seabream; condition factor; food deprivation; growth hormone; growth hormone receptor; proximate composition in liver; radioreceptor binding; ribonuclease protection assay; triiodothyronine
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Zoology; ZOOLOGY

Last updated on 2021-11-05 at 01:02