Apoptosis and expression of cytokines triggered by pyropheophorbide-a methyl ester-mediated photodynamic therapy in nasopharyngeal carcinoma cells
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香港中文大學研究人員

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摘要The photodynamic properties of pyropheophorbide-a methyl ester (MPPa), a semi-synthetic photosensitizer derived from chlorophyll a, were evaluated in a human nasopharyngeal carcinoma HONE-1 cell line. MPPa was non-toxic to the HONE-1. At the concentrations of 0.5-2 mu M, MPPa-mediated a drug dose-dependent photocytotoxicity in the HONE-1 cells. Confocal microscopy revealed a subcellular localization of MPPa in mitochondria and the Golgi apparatus. MPPa PDT-induced apoptosis was associated with the collapse of mitochondrial membrane potential, release of cytochrome c, the up-regulation of endoplasmic reticulum (ER) stress proteins (calnexin, Grp 94 and Grp78), and the activation of caspases-3 and -9. The photocytotoxicity was reduced by the corresponding specific caspase inhibitors. MPPa PDT-treated HONE-1 cells also up-regulated the gene expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) and beta-chemokines (MIP-1 beta, MPIF-1, and MPIF-2). These results suggest that the MPPa may be developed as a chlorophyll-based photosensitizer for the treatment of nasopharyngeal carcinoma. (C) 2006 Elsevier B.V. All rights reserved.
著者Li KM, Sun X, Koon HK, Leung WN, Fung MC, Wong RNS, Lung ML, Chang CK, Mak NK
期刊名稱Photodiagnosis and Photodynamic Therapy
出版年份2006
月份12
日期1
卷號3
期次4
出版社ELSEVIER SCIENCE BV
頁次247 - 258
國際標準期刊號1572-1000
電子國際標準期刊號1873-1597
語言英式英語
關鍵詞Apoptosis; Mitochondrial membrane potential; Nasopharyngeal carcinoma; Photodynamic therapy; Pyropheophorbide-a methyl ester
Web of Science 學科類別Oncology; ONCOLOGY

上次更新時間 2020-29-11 於 01:48