Anti-dermatophytic activity of bakuchiol: In vitro mechanistic studies and in vivo tinea pedis-inhibiting activity in a guinea pig model
Publication in refereed journal


引用次數
替代計量分析
.

其它資訊
摘要Bakuchiol was an active antifungal compound isolated from Psoraleae Fructus by means of bioassay-guided fractionation in our previous study. The present work aimed to investigate the underlying mechanisms and the therapeutic effect of bakuchiol in Trichophyton mentagrophytes-induced tinea pedis. After exposure to bakuchiol at 0.25-fold, 0.5-fold and 1-fold of minimum inhibitory concentration (MIC) (3.91 mu g/ml) for 24 h, the fungal conidia of T. mentagrophytes demonstrated a significant dose-dependent increase in membrane permeability. Moreover, bakuchiol at 1-fold MIC elicited a 187% elevation in reactive oxygen species (ROS) level in fungal cells after a 3-h incubation. However, bakuchiol did not induce DNA fragmentation. In a guinea pig model of tinea pedis, bakuchiol at 1%, 5% or 10% (w/w) concentration in aqueous cream could significantly reduce the fungal burden of infected feet (p < 0.01-0.05). In conclusion, this is the first report to demonstrate that bakuchiol is effective in relieving tinea pedis and in inhibiting the growth of the dermatophyte T. mentagrophytes by increasing fungal membrane permeability and ROS generation, but not via induction of DNA fragmentation. (C) 2014 Elsevier GmbH. All rights reserved.
著者Lau KM, Wong JH, Wu YO, Cheng L, Wong CW, To MH, Lau CP, Yew DTW, Leung PC, Fung KP, Hui M, Ng TB, Lau CBS
期刊名稱Phytomedicine
出版年份2014
月份6
日期15
卷號21
期次7
出版社ELSEVIER GMBH, URBAN & FISCHER VERLAG
頁次942 - 945
國際標準期刊號0944-7113
電子國際標準期刊號1618-095X
語言英式英語
關鍵詞Antifungal; Bakuchiol; Membrane permeability; ROS production; Tinea pedis; Trichophyton mentagrophytes
Web of Science 學科類別Chemistry, Medicinal; CHEMISTRY, MEDICINAL; Integrative & Complementary Medicine; INTEGRATIVE & COMPLEMENTARY MEDICINE; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Plant Sciences; PLANT SCIENCES

上次更新時間 2021-23-01 於 02:07