Genome-wide survey by ChIP-seq reveals YY1 regulation of lincRNAs in skeletal myogenesis
Publication in refereed journal


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摘要Skeletal muscle differentiation is orchestrated by a network of transcription factors, epigenetic regulators, and non-coding RNAs. The transcription factor Yin Yang 1 (YY1) silences multiple target genes in myoblasts (MBs) by recruiting Ezh2 (Enhancer of Zeste Homologue2). To elucidate genome-wide YY1 binding in MBs, we performed chromatin immunoprecipitation (ChIP)-seq and found 1820 specific binding sites in MBs with a large portion residing in intergenic regions. Detailed analysis demonstrated that YY1 acts as an activator for many loci in addition to its known repressor function. No significant co-occupancy was found between YY1 and Ezh2, suggesting an additional Ezh2-independent function for YY1 in MBs. Further analysis of intergenic binding sites showed that YY1 potentially regulates dozens of large intergenic non-coding RNAs (lincRNAs), whose function in myogenesis is under-explored. We characterized a novel muscle-associated lincRNA (Yam-1) that is positively regulated by YY1. Yam-1 is downregulated upon differentiation and acts as an inhibitor of myogenesis. We demonstrated that Yam-1 functions through in cis regulation of miR-715, which in turn targets Wnt7b. Our findings not only provide the first genome-wide picture of YY1 association in muscle cells, but also uncover the functional role of lincRNA Yam-1.
著者Lu LN, Sun K, Chen XN, Zhao Y, Wang LJ, Zhou L, Sun H, Wang HT
期刊名稱EMBO Journal
出版年份2013
月份10
日期2
卷號32
期次19
出版社NATURE PUBLISHING GROUP
頁次2575 - 2588
國際標準期刊號0261-4189
語言英式英語
關鍵詞ChIP-seq; lincRNA; myogenesis; PRC2; Yam-1; YY1
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

上次更新時間 2021-25-02 於 01:34