An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
Publication in refereed journal


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摘要Arabidopsis thaliana is a widely used model plant for plant biology research. Under traditional agar-plate culture system (TPG, traditional plant-growing), both plant shoots and roots are exposed to illumination, and roots are grown in sucrose-added medium. This is not a natural environment for the roots and may cause artifact responses. We have developed an improved agar-plate culture system (IPG, improved plant-growing) where shoots are illuminated but roots are grown in darkness without sucrose addition. Compared to TPG, IPG produced plants with significantly less total root length, lateral root length and root hair density, although their primary roots were longer. Root gravitropism, PIN2 (an auxin efflux carrier) abundance, H+ efflux or Ca2+ influx in root apexes, were weaker in IPG-grown roots than those in TPG-grown roots. We conclude that IPG offers a more natural way to study the root growth and response of Arabidopsis thaliana.
著者Xu WF, Ding GC, Yokawa K, Baluska F, Li QF, Liu YG, Shi WM, Liang JS, Zhang JH
期刊名稱Scientific Reports
詳細描述To ORKTS: 229. Xu W, Ding G, Yokawa K, Baluška F, Li Q, Liu Y, Shi W, Liang J, Zhang J. 2013. An improved agar-plate method for studying root gro
出版年份2013
月份2
日期14
卷號3
出版社NATURE PUBLISHING GROUP
國際標準期刊號2045-2322
電子國際標準期刊號2045-2322
語言英式英語
Web of Science 學科類別Multidisciplinary Sciences; MULTIDISCIPLINARY SCIENCES; Science & Technology - Other Topics

上次更新時間 2020-18-11 於 01:06