Durable Mesenchymal Stem Cell Labelling by Using Polyhedral Superparamagnetic Iron Oxide Nanoparticles
Publication in refereed journal


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摘要Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO2 and SPIO@SiO2-NH2). Surface modification of the small polyhedral silica-coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface-modified nanoparticles (SPIO@SiO2-NH2) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling Of SPIO@SiO2 and SPIO@SiO2-NH2 did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO2-NH2 nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO2-NH2 nanoparticle-labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long-lasting, durable MRI labelling efficacy after 8-12 weeks.
著者Wang HH, Wang YXJ, Leung KCF, Au DWT, Xuan SH, Chak CP, Lee SKM, Sheng H, Zhang G, Qin L, Griffith JE, Ahuja AT
期刊名稱Chemistry - A European Journal
出版年份2009
月份1
日期1
卷號15
期次45
出版社Wiley: 12 months
頁次12417 - 12425
國際標準期刊號0947-6539
電子國際標準期刊號1521-3765
語言英式英語
關鍵詞contrast agents; diagnostic agents; iron; magnetic properties; nanoparticles
Web of Science 學科類別Chemistry; Chemistry, Multidisciplinary; CHEMISTRY, MULTIDISCIPLINARY

上次更新時間 2021-13-01 於 00:35