Gi-coupled prostanoid receptors are the likely targets for COX-1-generated prostanoids in rat pheochromocytoma (PC12) cells
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AbstractCyclooxygenase-l (COX-I) behaves as a delayed response gene in rat pheochromocytoma (PC12) cells exposed to nerve growth factor (NGF). To investigate the possible targets for COX-1 generated prostanoids in the early stages of neuronal differentiation, we have examined the expression of prostanoid receptors by PC12 cells using functional assays. Prostanoid receptor-specific agonists failed to activate adenylyl cyclase in undifferentiated and NGF-treated PC12 cells: neither did they stimulate phospholipase C activity. EP3 receptor agonists and PGF(2 alpha) were the only active ligands, able to inhibit forskolin-stimulated adenylyl cyclase activity. PC] 2 cells expressed EP3 and FP receptor mRNA, but only the responses to EP3 receptor agonists were inhibited by the EP3 receptor antagonist ONO-AE3-240. The functional role of NGF-stimulated COX-1 remains to be determined since we found no strong evidence of a role for EP3 receptors in the morphological changes induced by NGF during the early stages of differentiation of PC12 cells. (C) 2009 Elsevier Ltd. All rights reserved.
All Author(s) ListYung HS, Chow KBS, Lai KH, Wise H
Journal nameProstaglandins, Leukotrienes and Essential Fatty Acids
Year2009
Month7
Day1
Volume Number81
Issue Number1
PublisherElsevier
Pages65 - 71
ISSN0952-3278
eISSN1532-2823
LanguagesEnglish-United Kingdom
KeywordsNeuronal differentiation; NGF; PC12 cells; Prostanoid receptors
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY; Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM

Last updated on 2020-02-07 at 03:42