Preliminary studies on LED-activated pyropheophorbide-alpha methyl ester killing cisplatin-resistant ovarian carcinoma cells
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AbstractIn the present study, a novel LED source was applied for activating pyropheophorbids-a methyl ester (MPPa) in cisplatin-resistant ovarian cell line COC1/DDP cells. MPPa concentration was 2 mu M and light energy from 0.125-8 J/cm(2). Cytotoxicity was investigated 24 h using MTT reduction assay and light microscopy after treatment. Cellular ultrastructure was observed using transmission electron microscopy (TEM) and nuclear chromatin by fluorescent microscope with Hoechst33258 staining. MTT reduction assay showed that the cytotoxicity of LED-activated MPPa in the COC1/DDP cells increased along with the light dose of LED source and LED-activated MPPa resulted in light-dependent cytotoxicity. The observations from light microscopy reinforced the above results. TEM showed that necrotic cells with the disruption of karyotheca, karyorrhexis, and karyolysis of nucleus and apoptotic cells, especially the apoptotic body, can be seen post LED-activated MPPa. Hoechst33258 staining showed that condensation of chromatin and nuclear fragmentations could be found in many treated cells and some of them formed the structure of apoptotic bodies when COC1/DDP cells were exposed to 2 mu M MPPa for 20 h and then 1 J/cm(2) irradiation of LED source. The findings demonstrated that the novel LED source could efficiently activated MPPa and LED-activated MPPa could significantly kill cisplatin-resistant ovarian cell line COC1/DDP cells through two major pathways including necrosis and apoptosis, suggesting that LED is a novel and efficient light source and LED-activated MPPa might be potential therapeutic modality for treating cisplatin-resistant ovarian carcinoma.
All Author(s) ListTan Y, Xu CS, Xia XS, Yu HP, Bai DQ, He Y, Xu J, Wang P, Wang XN, Leung AWN
Journal nameLaser Physics
Detailed descriptionTo ORKTS: LEUNG Wing Nang is Co-corresponding author.
Volume Number19
Issue Number5
Pages1045 - 1049
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesOptics; OPTICS; Physics; Physics, Applied; PHYSICS, APPLIED

Last updated on 2020-20-11 at 01:20