Advanced proteomic technologies for cancer biomarker discovery
Publication in refereed journal


摘要Proteomic technologies have experienced major improvements in recent years. Such advances have facilitated the discovery of potential tumor markers with improved sensitivities and specificities for the diagnosis, prognosis and treatment monitoring of cancer patients. This review will focus on four state-of-the-art proteomic technologies, namely 2D difference gel electrophoresis, MALDI imaging mass spectrometry, electron transfer dissociation mass spectrometry and reverse-phase protein array. The major advancements these techniques have brought about and examples of their applications in cancer biomarker discovery will be presented in this review, so that readers can appreciate the immense progress in proteomic technologies from 1997 to 2008. Finally, a summary will be presented that discusses current hurdles faced by proteomic researchers, such as the wide dynamic range of protein abundance, standardization of protocols and validation of cancer biomarkers, and a 5-year view of potential solutions to such problems will be provided.
著者Wong SCC, Chan CML, Ma BBY, Lam MYY, Choi GCG, Au TCC, Chan ASK, Chan ATC
期刊名稱Expert Review of Proteomics
出版社Taylor & Francis: STM, Behavioural Science and Public Health Titles - No Open Select
頁次123 - 134
關鍵詞2D difference gel electrophoresis; cancer biomarker; electron transfer dissociation; MALDI imaging mass spectrometry; proteomics; reverse-phase protein array
Web of Science 學科類別Biochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology

上次更新時間 2020-18-11 於 00:44