Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses
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AbstractFour IgG(1K) monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1. 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC(90) neutralizing titre range was >1600-51,200, and with the microneutralization was 80 > 10,240, MAb 1C1 showed strong neutralizing activities in both assays. All four mAbs reacted specifically to the immunogen by immunohistochemical staining and to A/Hong Kong/483/1997 (H5N1) and A/Thailand/1(KAN-1)/2004 (H5N1)-infected MDCK cells by immunofluorescence. ELISA titrations of the mAbs showed specificity for H5N1 haemagglutinin (HA) and no cross-reactivity to 15 other Influenza A subtypes. Only mAbs 1C1 and the non-neutralizing 1F7 reacted with HA(1), the cleaved subunit of HA, by Western blot. These results suggest that the mAbs recognize distinct or overlapping epitopes and will be useful reagents for construction of specific rapid point-of-care assays or for therapeutic use. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
All Author(s) ListOh S, Selleck P, Temperton NJ, Chan PKS, Capecchi B, Manavis J, Higgins G, Burrell CJ, Kok T
Name of ConferenceInternational Conference on Policy Modeling-EcoMod 2005
Start Date of Conference29/06/2005
End Date of Conference02/07/2005
Place of ConferenceIstanbul
Journal nameJournal of Virological Methods
Volume Number157
Issue Number2
Pages161 - 167
LanguagesEnglish-United Kingdom
KeywordsH5N1; Haemagglutinin; Influenza; Monoclonal antibodies; Neutralization; Pseudotype
Web of Science Subject CategoriesBiochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Virology; VIROLOGY

Last updated on 2021-19-09 at 00:24