HO-1-u-1 model for screening sublingual drug delivery-Influence of pH, osmolarity and permeation enhancer
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香港中文大學研究人員

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摘要HO-1-u-1 (Ueda-1) is a human tumor cell line established from human sublingual squamous cell carcinoma. In previous study, HO-1-u-1 cell line was grown on cell culture inserts and utilized as in vitro model for screening sublingual drug delivery. The aim of current study was to further investigate the effects of pH, osmolarity and permeation enhancer, sodium glycodeoxycholate (GDC) on the permeability of three beta-blockers with different lipophilicities. The cytotoxicity was evaluated by MTS/PES assay. The permeability studies were carried out using the cell culture model and compared with that obtained from fresh porcine sublingual mucosa. The results showed the enhancement effects caused by pH, osmolarity and GDC were highly lipophilicity-dependent and in the order atenolol > metoprolol > propranolol. The apparent permeability coefficients (P(app)) of all the three beta-blockers were significantly increased by increasing pH. However, less enhancing effects were observed by non-physiological osmolarity or the presence of GDC in permeability study using both cell culture and porcine sublingual mucosa. The present results suggested that the HO-1-u-1 cell culture model maybe a useful and effective in vitro model for evaluating the enhancement effects and mechanism in sublingual drug delivery. (C) 2008 Elsevier B.V. All rights reserved.
著者Wang YF, Zuo Z, Chow MSS
期刊名稱International Journal of Pharmaceutics
出版年份2009
月份3
日期31
卷號370
期次1-2
出版社Elsevier
頁次68 - 74
國際標準期刊號0378-5173
電子國際標準期刊號1873-3476
語言英式英語
關鍵詞beta-Blocker; Enhancer; HO-1-u-1; Osmolarity; pH; Sublingual delivery
Web of Science 學科類別Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

上次更新時間 2022-16-01 於 00:09