Effect of hyperoxidized guanine on DNA primer-template structures: Spiroiminodihydantoin leads to strand slippage
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AbstractOxidation of guanine in DNA can lead to mutagenic lesions such as 7-hydro-8-oxoguanine (oG). Upon further oxidation, a more mutagenic lesion, spirominodihydantoin (Sp), can occur. In this study, nuclear magnetic resonance (NMR) investigations were performed to determine the structural features of DNA primer-template models with 5'-GG, 5'-G(oG), 5'-G(Sp) and 5'-T(Sp) templates, that mimic the situation in which the downstream G of the template has been oxidized to oG or hyperoxidized to Sp. Our results show that misalignment occurs only in the 5'-G(Sp) and 5'-T(Sp) templates, providing structural insights into the observed differences in mutagenicity of Sp and oG during DNA replication. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
All Author(s) ListFenn D, Chi LM, Lam SL
Journal nameFEBS Letters
Volume Number582
Issue Number30
PublisherWiley: 12 months
Pages4169 - 4175
LanguagesEnglish-United Kingdom
KeywordsDNA; Guanine; NMR; Spiroiminodihydantoin; Strand slippage
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

Last updated on 2021-19-09 at 00:18