Effect of 1-methyladenine on double-helical DNA structures
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AbstractMethylation at the N1 site of adenine leads to the formation of cytotoxic 1-methyladenine (m1A). Since the N1 site of adenine is involved in the hydrogen bonding of T A and A T Watson-Crick base pairs, it is expected that the pairing interactions will be disrupted upon 1-methylation. In this study, high-resolution NMR investigations were performed to determine the effect of m1A on double-helical DNA structures. Interestingly, instead of disrupting hydrogen bonding, we found that 1-methylation altered the T.A Watson-Crick base pair to T(anti) m1A(syn) Hoogsteen base pair, providing insights into the observed differences in AlkB-repair efficiency between dsDNA and ssDNA. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
All Author(s) ListYang H, Zhan YQ, Fenn D, Chi LM, Lam SL
Journal nameFEBS Letters
Year2008
Month5
Day14
Volume Number582
Issue Number11
PublisherWiley: 12 months
Pages1629 - 1633
ISSN0014-5793
eISSN1873-3468
LanguagesEnglish-United Kingdom
Keywords1-methylation; adenine; DNA; Hoogsteen base pair; NMR
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

Last updated on 2021-15-09 at 04:20