Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma
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AbstractDiethylnitrosamine (DEN) is a known carcinogen that can alkylate DNA molecules. In rats, DEN-induced hepatocellular carcinoma (HCC) model is well established. In this study, we used a two-dimensional differential gel electrophoresis (2D-DIGE) system and liquid chromatography/mass spectrometry/mass spectrometry to identify the differential expression protein profiles between the DEN-induced HCC and healthy liver cells. Western blotting and semiquantitative RT-PCR were used to further confirm the results. Seventeen differentially expressed spots were identified in DEN-induced HCC cells. Among all, the most prominent upregulated proteins include the members of the glutathione S-transferase super family, aldo-keto reductase superfamily and proteins involved in the response to oxidative stress. Downregulation was observed in 2 proteins that were known to contribute to hepatic dysfunction. This study provides the first comprehensive protein profiling of the DEN-induced HCC in rats. This model simulates the differential protein expression of human HCC and may be useful for further understanding the mechanism of HCC tumorigenesis. (C) 2008 Wiley-Liss, Inc.
All Author(s) ListQi YT, Chen X, Chan CY, Li D, Yuan CG, Yu F, Lin MC, Yew DT, Kung HF, Lai L
Journal nameInternational Journal of Cancer
Volume Number122
Issue Number12
Pages2682 - 2688
LanguagesEnglish-United Kingdom
Keywordsdiethylnitrosamine; glutathione S-transferase; hepatocellular carcinoma; two-dimensional differential gel electrophoresis
Web of Science Subject CategoriesOncology; ONCOLOGY

Last updated on 2020-26-11 at 00:48