Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry
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AbstractThe current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 mu L plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C-18 reversed-phase HPLC column (150 x 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples. Copyright (c) 2007 John Wiley & Sons, Ltd.
All Author(s) ListLi Z, Hu Y, Ming LW, Chun CM, Ping PY, Ge L, Tao WY, Zhong Z, Fan HY
Journal nameBiomedical Chromatography
Year2008
Month4
Day1
Volume Number22
Issue Number4
PublisherWiley: 12 months
Pages414 - 420
ISSN0269-3879
eISSN1099-0801
LanguagesEnglish-United Kingdom
KeywordsAlzheimer's disease; bis(7)-tacrine; LC-MS-MS; rat plasma
Web of Science Subject CategoriesBiochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-05-08 at 03:28