Antiviral and anti-proliferative glycoproteins from the rhizome of Smilax glabra roxb (Liliaceae)
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AbstractThe glycoproteins possessing antiviral and anti-proliferative activities were isolated from the Chinese medicinal herb Smilax glabra (known as tufuling), by extraction with 0.2 M NaCl, ammonium sulfate precipitation, fetuin-agarose affinity chromatography and get filtration. The molecular mass of the fetuin-binding glycoprotein (designated SGPF2) was estimated to be about 58 kDa, with a major protein subunit of 26 kDa. The non-fetuin binding glycoproteins (in the unadsorbed fraction) were further separated into 5 different subtractions (SGPF1a-SGPF1e) with anion-exchange chromatography, all of which also contained the major band at 26 kDa. All the isolated proteins of 26 kDa had similar N-terminal amino acid sequences, implying that they were probably the isoforms originated putatively from a multigene family with different binding affinity and ionic strength. The glycoprotein SGPF2 exhibited antiviral activity against respiratory syncytial virus (RSV) with a median inhibitory concentration (IC50) of 62.5 mu g/ml and Herpes simplex virus type 1 (HSV-1) had an IC50 of 31.3 mu g/ml. The glycoprotein potencies for antiviral activity appeared to depend on the molecules' binding affinity for fetuin, that is, the fetuin-binding protein was more potent than the non-fetuin binding proteins. Further examination revealed that these glycoproteins also had the ability to suppress the proliferation of MCF-7 cells. The possible mechanism of anti-proliferative action as analyzed by DNA flow cytometry indicated that they could induce apoptosis mediated via sub-G(1) phase of the MCF-7 cell cycle. For example, there was an increase by 75.8% of the control level of apoptosis after incubation with SGPF1a.
All Author(s) ListOoi LSM, Wong EYL, Chiu LCM, Sun SSM, Ooi VEC
Journal nameAmerican Journal of Chinese Medicine
Year2008
Month1
Day1
Volume Number36
Issue Number1
PublisherWorld Scientific Publishing
Pages185 - 195
ISSN0192-415X
eISSN1793-6853
LanguagesEnglish-United Kingdom
Keywordsanti-proliferative; anti-viral; apoptosis; fetuin-binding protein; glycoprotein; Smilax glabra
Web of Science Subject CategoriesGeneral & Internal Medicine; Integrative & Complementary Medicine; INTEGRATIVE & COMPLEMENTARY MEDICINE; Medicine, General & Internal; MEDICINE, GENERAL & INTERNAL

Last updated on 2020-03-07 at 01:57