Activation of Transient Receptor Potential Vanilloid 3 Channel Suppresses Adipogenesis
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摘要The present study shows that activation of the transient receptor potential vanilloid 3 channel (TRPV3) suppresses adipocyte differentiation. We also found that a major functional catechin compound in green tea and cocoa, (-)-epicatechin, exerts antiadipogenic effects in the adipocytes through direct activation of TRPV3. TRPV3 was detected in the 3T3-L1 adipocytes using immunohistochemistry and semiquantitative PCR. TRPV3 activation by activators (-)- epicatechin and diphenylborinic anhydride was determined using live cell fluorescent Ca2+ imaging and patch-clamp electrophysiology. Using RNA interference, immunoblotting, and Oil red O staining, we found that the TRPV3 agonists prevented adipogenesis by inhibiting the phosphorylation of insulin receptor substrate 1, the downstream phosphoinositide 3-kinase/Akt/forkhead box protein O1 axis, and the expression of the adipogenic genes peroxisome proliferator-activated receptor gamma and CCAAT/enhancer-binding protein alpha. TRPV3 overexpression hindered adipogenesis in the 3T3-L1 cells. In vivo studies showed that chronictreatment with the TRPV3 activators prevented adipogenesis and weight gain in the mice fed on high-fat diets. Moreover, TRPV3 expression was reduced in the visceral adipose tissue from mice fed on high-fat diets andobese(ob/ob) and diabetic (db/m(+)) mice. In conclusion, our study illustrates the antiadipogenic role of TRPV3 in the adipocytes.
著者Cheung SY, Huang Y, Kwan HY, Chung HY, Yao XQ
期刊名稱Endocrinology
詳細描述To ORKTS: doi: 10.1210/en.2014-1831
出版年份2015
月份6
日期1
卷號156
期次6
出版社Endocrine Society
頁次2074 - 2086
國際標準期刊號0013-7227
電子國際標準期刊號1945-7170
語言英式英語
Web of Science 學科類別Endocrinology & Metabolism

上次更新時間 2021-16-01 於 23:41