Activation of Transient Receptor Potential Vanilloid 3 Channel Suppresses Adipogenesis
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AbstractThe present study shows that activation of the transient receptor potential vanilloid 3 channel (TRPV3) suppresses adipocyte differentiation. We also found that a major functional catechin compound in green tea and cocoa, (-)-epicatechin, exerts antiadipogenic effects in the adipocytes through direct activation of TRPV3. TRPV3 was detected in the 3T3-L1 adipocytes using immunohistochemistry and semiquantitative PCR. TRPV3 activation by activators (-)- epicatechin and diphenylborinic anhydride was determined using live cell fluorescent Ca2+ imaging and patch-clamp electrophysiology. Using RNA interference, immunoblotting, and Oil red O staining, we found that the TRPV3 agonists prevented adipogenesis by inhibiting the phosphorylation of insulin receptor substrate 1, the downstream phosphoinositide 3-kinase/Akt/forkhead box protein O1 axis, and the expression of the adipogenic genes peroxisome proliferator-activated receptor gamma and CCAAT/enhancer-binding protein alpha. TRPV3 overexpression hindered adipogenesis in the 3T3-L1 cells. In vivo studies showed that chronictreatment with the TRPV3 activators prevented adipogenesis and weight gain in the mice fed on high-fat diets. Moreover, TRPV3 expression was reduced in the visceral adipose tissue from mice fed on high-fat diets andobese(ob/ob) and diabetic (db/m(+)) mice. In conclusion, our study illustrates the antiadipogenic role of TRPV3 in the adipocytes.
All Author(s) ListCheung SY, Huang Y, Kwan HY, Chung HY, Yao XQ
Journal nameEndocrinology
Detailed descriptionTo ORKTS: doi: 10.1210/en.2014-1831
Volume Number156
Issue Number6
PublisherEndocrine Society
Pages2074 - 2086
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesEndocrinology & Metabolism

Last updated on 2021-21-10 at 00:41