Tissue-specific transcriptome assemblies of the marine medaka Oryzias melastigma and comparative analysis with the freshwater medaka Oryzias latipes
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AbstractBackground: The marine medaka Oryzias melastigma has been demonstrated as a novel model for marine ecotoxicological studies. However, the lack of genome and transcriptome reference has largely restricted the use of O. melastigma in the assessment of in vivo molecular responses to environmental stresses and the analysis of biological toxicity in the marine environment. Although O. melastigma is believed to be phylogenetically closely related to Oryzias latipes, the divergence between these two species is still largely unknown. Using Illumina high-throughput RNA sequencing followed by de novo assembly and comprehensive gene annotation, we provided transcriptomic resources for the brain, liver, ovary and testis of O. melastigma. We also investigated the possible extent of divergence between O. melastigma and O. latipes at the transcriptome level.
All Author(s) ListLai KP, Li JW, Wang SY, Chiu JMY, Tse A, Lau K, Lok S, Au DWT, Tse WKF, Wong CKC, Chan TF, Kong RYC, Wu RSS
Journal nameBMC Genomics
Volume Number16
LanguagesEnglish-United Kingdom
KeywordsDe novo assembly; High-throughput RNA sequencing; Marine and freshwater; Medaka; Transcriptome assembly
Web of Science Subject CategoriesBiotechnology & Applied Microbiology; Genetics & Heredity

Last updated on 2020-10-08 at 02:09