B cell CLL/lymphoma 6 member B inhibits hepatocellular carcinoma metastases in vitro and in mice
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摘要B cell CLL/lymphoma 6 member B (BCL6B) is a novel tumor suppressor silenced in human cancer. In this study, we investigated the functional role and underlying mechanisms of BCL6B in hepatocellular carcinoma (HCC). BCL6B was expressed in normal HCC tissues, but its expression was suppressed in 6 out of 9 HCC cell lines. Loss of BCL6B expression was associated with promoter hypermethylation. Ectopic expression of BCL6B in HepG2 and Huh7 cell lines inhibited colony formation (P<0.05), cell viability (P<0.01), and tumorigenicity in nude mice (P<0.05). BCL6B expression also induced apoptosis (P<0.05), an effect associated with activation of the caspase cascade and cleavage of PARP. Stable expression of BCL6B in MHCC97L cells suppressed cell migration (P<0.05) and invasion (P<0.05), and significantly reduced the incidence and severity of lung metastasis in an orthotopic HCC mouse model. The anti-metastatic effect of BCL6B was mediated by up-regulation of cell adhesion gene E-cadherin, OB-cadherin, HIV-1 Tat interactive protein 2, and transient receptor potential cation channel, subfamily M, member 1; and down-regulation of angiogenesis gene VEGFA. BCL6B functions as a tumor suppressor that inhibits HCC metastases in vitro and in vivo. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.
著者Wang J, Dong L, Xu LX, Chu ESH, Chen YC, Shen JY, Li XX, Wong CC, Sung JJY, Yu J
期刊名稱Cancer Letters
出版年份2014
月份12
日期28
卷號355
期次2
出版社Elsevier
頁次192 - 200
國際標準期刊號0304-3835
電子國際標準期刊號1872-7980
語言英式英語
關鍵詞B cell CLL/Iymphoma 6 member B; Epigenetic alteration; Hepatocellular carcinoma; Tumor metastasis; Tumor suppressive gene
Web of Science 學科類別Oncology

上次更新時間 2021-11-05 於 00:28