Abnormal Response of the Proliferation and Differentiation of Growth Plate Chondrocytes to Melatonin in Adolescent Idiopathic Scoliosis
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AbstractAbnormalities in the melatonin signaling pathway and the involvement of melatonin receptor MT2 have been reported in patients with adolescent idiopathic scoliosis (AIS). Whether these abnormalities were involved in the systemic abnormal skeletal growth in AIS during the peripubertal period remain unknown. In this cross-sectional case-control study, growth plate chondrocytes (GPCs) were cultured from twenty AIS and ten normal control subjects. Although the MT2 receptor was identified in GPCs from both AIS and controls, its mRNA expression was significantly lower in AIS patients than the controls. GPCs were cultured in the presence of either the vehicle or various concentrations of melatonin, with or without the selective MT2 melatonin receptor antagonist 4-P-PDOT (10 mu M). Then the cell viability and the mRNA expression of collagen type X (COLX) and alkaline phosphatase (ALP) were assessed by MTT and qPCR, respectively. In the control GPCs, melatonin at the concentrations of 1, 100 nM and 10 mu M significantly reduced the population of viable cells, and the mRNA level of COLX and ALP compared to the vehicle. Similar changes were not observed in the presence of 4-P-PDOT. Further, neither proliferation nor differentiation of GPCs from AIS patients was affected by the melatonin treatment. These findings support the presence of a functional abnormality of the melatonin signaling pathway in AIS GPCs, which might be associated with the abnormal endochondral ossification in AIS patients.
All Author(s) ListWang WWJ, Man GCW, Wong JH, Ng TB, Lee KM, Ng BKW, Yeung HY, Qiu Y, Cheng JCY
Journal nameInternational Journal of Molecular Sciences
Detailed descriptionTo ORKTS: doi: 10.3390/ijms150917100
Year2014
Month9
Day1
Volume Number15
Issue Number9
PublisherMDPI AG
Pages17100 - 17114
ISSN1661-6596
LanguagesEnglish-United Kingdom
Keywordsantagonist; chondrocytes; differentiation; dysfunction; growth plate; idiopathic scoliosis; melatonin; MT2 receptor; proliferation
Web of Science Subject CategoriesBiochemistry & Molecular Biology; Chemistry; Chemistry, Multidisciplinary

Last updated on 2020-02-06 at 00:42