Application of a target enrichment-based next-generation sequencing protocol for identification and sequence-based prediction of pneumococcal serotypes
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AbstractBackground: The use of whole-genome sequencing in microbiology at a diagnostic level, although feasible, is still limited by the expenses associated and by the complex bioinformatics pipelines in data analyses. We describe the use of target enrichment-based next-generation sequencing for pneumococcal identification and serotyping as applied to the polysaccharide 23 valent vaccine serotypes as an affordable alternative to whole genome sequencing.
All Author(s) ListLiyanapathirana V, Ang I, Tsang D, Fung K, Ng TK, Zhou HK, Ip M
Journal nameBMC Microbiology
Year2014
Month3
Day10
Volume Number14
PublisherBIOMED CENTRAL LTD
ISSN1471-2180
LanguagesEnglish-United Kingdom
KeywordsNext generation sequencing (NGS); Serotyping; Streptococcus pneumoniae
Web of Science Subject CategoriesMicrobiology; MICROBIOLOGY

Last updated on 2021-23-02 at 00:17