Application of a target enrichment-based next-generation sequencing protocol for identification and sequence-based prediction of pneumococcal serotypes
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摘要Background: The use of whole-genome sequencing in microbiology at a diagnostic level, although feasible, is still limited by the expenses associated and by the complex bioinformatics pipelines in data analyses. We describe the use of target enrichment-based next-generation sequencing for pneumococcal identification and serotyping as applied to the polysaccharide 23 valent vaccine serotypes as an affordable alternative to whole genome sequencing.
著者Liyanapathirana V, Ang I, Tsang D, Fung K, Ng TK, Zhou HK, Ip M
期刊名稱BMC Microbiology
出版年份2014
月份3
日期10
卷號14
出版社BIOMED CENTRAL LTD
國際標準期刊號1471-2180
語言英式英語
關鍵詞Next generation sequencing (NGS); Serotyping; Streptococcus pneumoniae
Web of Science 學科類別Microbiology; MICROBIOLOGY

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