Cerebral vasodilator properties of Danshen and Gegen: A study of their combined efficacy and mechanisms of actions
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AbstractDanshen and Gegen are two commonly used Chinese herbal medicines for treatment of cardiovascular diseases. The aim of the present study was to elucidate the combination effects, of these two herbs on cerebral vascular tone and their underlying mechanisms of actions. Basilar artery rings were obtained from rats and precontracted with U46619. Cumulative administrations of aqueous extracts of Danshen, Gegen, or the two herbs combined (DG; ratio 7:3) produced concentration-dependent relaxation of the artery rings. Statistical analysis on these findings produced a combination index (CI) of 1.041 at ED50, which indicates the two herbs produced additive vasodilator effects when used as a combined decoction. Removal of the endothelium had no effect on the vasodilator properties of Danshen, Gegen, and DG. However, their maximum effects (Imax) were significantly blunted by a K-ATP channel inhibitor glibenclamide, a non-selective K+ channel inhibitor tetraethylammonium (TEA), and by a combination of K+ channel inhibitors (glibenclamide+TEA+iberiotoxin+4-aminopyridine+barium chloride). In addition, Danshen, Gegen, and DG produced augmentation of K-ATP currents and inhibited Ca2+ influx in vascular smooth muscle cells isolated from rat basilar arteries. Furthermore, these agents inhibited CaCl2-induced contraction in the artery rings. In conclusion, the present study showed that Danshen and Gegen produced additive vasodilator effects on rat cerebral basilar arteries. These effects were independent of endothelium-derived relaxant factors (EDRF), but required the opening of K-ATP channels and inhibition of Ca2+ influx in the vascular smooth muscle cells. It is suspected that the cerebral vasodilator effects of Danshen and Gegen produced either on their own or in combination, can help patients with obstructive cerebrovascular diseases. (C) 2013 Elsevier GmbH. All rights reserved.
All Author(s) ListDeng Y, Ng ESK, Kwan YW, Lau CBS, Cheung DWS, Koon JCM, Zhang Z, Zuo Z, Leung PC, Fung KP, Lam FFY
Journal namePhytomedicine
Volume Number21
Issue Number4
Pages391 - 399
LanguagesEnglish-United Kingdom
KeywordsBasilar artery; Combination index; Danshen; Gegen; Stroke; Vasodilatation
Web of Science Subject CategoriesChemistry, Medicinal; CHEMISTRY, MEDICINAL; Integrative & Complementary Medicine; INTEGRATIVE & COMPLEMENTARY MEDICINE; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Plant Sciences; PLANT SCIENCES

Last updated on 2020-17-10 at 00:01