Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata
Publication in refereed journal


摘要Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked alpha-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), macrophage inhibitory protein-1 alpha (MIP-1 alpha), macrophage inhibitory protein-1 beta (MIP-1 beta), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer. (C) 2013 Elsevier Ltd. All rights reserved.
著者Cheng BH, Chan JYW, Chan BCL, Lin HQ, Han XQ, Zhou XL, Wan DCC, Wang YF, Leung PC, Fung KP, Bik-San Lau C
期刊名稱Carbohydrate Polymers
頁次244 - 249
關鍵詞HCP-2; Houttuynia cordata; Immunomodulation; Pectic polysaccharide; Structural characterization
Web of Science 學科類別Chemistry; Chemistry, Applied; CHEMISTRY, APPLIED; Chemistry, Organic; CHEMISTRY, ORGANIC; Polymer Science; POLYMER SCIENCE

上次更新時間 2021-06-03 於 23:15