Development and validation of a sensitive method for simultaneous determination of rosuvastatin and N-desmethyl rosuvastatin in human plasma using liquid chromatography/negative electrospray ionization/tandem mass spectrometry
Publication in refereed journal

Times Cited
Web of Science14WOS source URL (as at 14/09/2021) Click here for the latest count
Altmetrics Information

Other information
AbstractA sensitive and specific liquid chromatography tandem mass spectrometric method was developed and validated for the simultaneous determination of rosuvastatin (ROS) and N-desmethyl rosuvastatin (NOR-ROS) in human plasma using deuterium-labeled internal standards. The plasma samples were prepared using liquid-liquid extraction with diethyl ether. Chromatographic separation was accomplished on an Xterra MS C-18 column. The mobile phase consisted of a gradient mixture of 15 mu mol/L ammonium acetate in water and in methanol, maintained at a flow rate of 0.4mL/min. Mass spectrometric detection was carried out in negative electrospray ionization mode and monitored by quantification and qualification transitions for each analyte. Using 300 L plasma samples, the lower limits of quantification of ROS and NOR-ROS were 0.05 and 0.02 mu g/L respectively. The linearity of ROS and NOR-ROS ranged from 0.05 to 42 and 0.02 to 14 mu g/L respectively. The relative standard deviations of ROS and NOR-ROS were <13 and 9%, respectively, while the deviations from expected values were within -4.7-9.8 and -5.2-4.6%, respectively. The present method offered high sensitivity and was successfully applied to a 24h pharmacokinetic study of ROS and NOR-ROS in healthy subjects receiving a single dose of 10mg ROS. Copyright (c) 2013 John Wiley & Sons, Ltd.
All Author(s) ListLee HK, Ho CS, Hu M, Tomlinson B, Wong CK
Journal nameBiomedical Chromatography
Detailed descriptionEpub 2013 May 30\n\nTo ORKTS: Published
Volume Number27
Issue Number11
PublisherWiley: 12 months
Pages1369 - 1374
LanguagesEnglish-United Kingdom
KeywordsHMG-CoA reductase inhibitor; LC; MS; N-desmethyl rosuvastatin; pharmacokinetics; rosuvastatin
Web of Science Subject CategoriesBiochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2021-15-09 at 00:48