Resolving antibody-peptide complexes with different ligand stoichiometries reveals a marked affinity enhancement through multivalency
Publication in refereed journal

香港中文大學研究人員

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摘要Natural antibodies adopt multivalent constructs to effect superior binding affinities with their antigens. Notwithstanding that the structure of antibodies have been well understood, how antibodies harness multivalency effect to achieve superior binding affinity toward the antigens still remains unclear. Such investigation is often hampered by the difficulty in resolving receptor-ligand complexes with different stoichiometries in the binding solution, especially when the ligand is a small molecule or a short peptide. Here we employed a unique anti-FLAG (TM) mAb M2 and FLAG (TM) peptides system, together with fluorescence detection coupled capillary electrophoresis (CE-FL) to reveal how M2 achieves exceptional high affinity with FLAG peptides through multivalency. Complexation of fluorescently labeled FLAG (TM) peptides with M2 leads to a pronounced mobility shift of the fluorescent peak in CE. Remarkably, CE-FL rendered a base-line separation of 1:1 and 1:2 M2 FLAG (TM) complexes, allowing the quantification of different M2 FLAG (TM) species. The quantitative analysis leads to a detailed dissection of the first (functional affinity) and second binding affinities (intrinsic affinity) between M2 and FLAG(1) peptide. The marked difference (10(3)-10(4) fold) between these two affinities indicated that multivalency effect plays a key role for M2 to achieve highly efficient binding to FLAG (TM) peptides. (C) 2013 Elsevier B.V. All rights reserved.
著者Wang JH, Jiang PJ, Qiu L, Wang CL, Xia J
期刊名稱Talanta
出版年份2013
月份10
日期15
卷號115
出版社Elsevier
頁次394 - 400
國際標準期刊號0039-9140
電子國際標準期刊號1873-3573
語言英式英語
關鍵詞Anti-FLAG antibody; Antibody-peptide interaction; Capillary electrophoresis; Ligand stoichiometry; Multivalency
Web of Science 學科類別Chemistry; Chemistry, Analytical; CHEMISTRY, ANALYTICAL

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