A lab-in-a-droplet bioassay strategy for centrifugal microfluidics with density difference pumping, power to disc and bidirectional flow control
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AbstractIn this paper, we present a lab-in-a-droplet bioassay strategy for a centrifugal microfluidics or lab-on-a-disc (LOAD) platform with three important advancements including density difference pumping, power to disc and bidirectional flow control. First, with the water based bioassay droplets trapped in a micro-channel filled with mineral oil, centrifugal force due to the density difference between the water and oil phases actuates droplet movement while the oil based medium remains stationary. Second, electricity is coupled to the rotating disc through a split-core transformer, thus enabling on-chip real-time heating in selected areas as desired and wireless programmable functionality. Third, an inertial mechanical structure is proposed to achieve bidirectional flow control within the spinning disc. The droplets can move back and forth between two heaters upon changing the rotational speed. Our platform is an essential and versatile solution for bioassays such as those involving DNA amplification, where localized temperature cycling is required. Finally, without the loss of generality, we demonstrate the functionality of our platform by performing real-time polymerase chain reaction (RT-PCR) in a linear microchannel made with PTFE (Teflon) micro-tubing.
All Author(s) ListWang GH, Ho HP, Chen QL, Yang AKL, Kwok HC, Wu SY, Kong SK, Kwan YW, Zhang XP
Journal nameLab on a Chip
Detailed descriptionTo ORKTS: doi: 10.1039/c31c50545f **Duplicate record P136086 deleted - Mar 28, 2014
Year2013
Month1
Day1
Volume Number13
Issue Number18
PublisherRoyal Society of Chemistry
Pages3698 - 3706
ISSN1473-0197
eISSN1473-0189
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; Chemistry; Chemistry, Multidisciplinary; CHEMISTRY, MULTIDISCIPLINARY; Nanoscience & Nanotechnology; NANOSCIENCE & NANOTECHNOLOGY; Science & Technology - Other Topics

Last updated on 2020-24-05 at 00:36