Three of Four GlnR Binding Sites Are Essential for GlnR-Mediated Activation of Transcription of the Amycolatopsis mediterranei nas Operon
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AbstractIn Amycolatopsis mediterranei U32, genes responsible for nitrate assimilation formed one operon, nasACKBDEF, whose transcription is induced by the addition of nitrate. Here, we characterized GlnR as a direct transcriptional activator for the nas operon. The GlnR-protected DNA sequences in the promoter region of the nas operon were characterized by DNase I footprinting assay, the previously deduced Streptomyces coelicolor double 22-bp GlnR binding consensus sequences comprising a1, b1, a2, and b2 sites were identified, and the sites were then mutated individually to test their roles in both the binding of GlnR in vitro and the GlnR-mediated transcriptional activation in vivo. The results clearly showed that only three GlnR binding sites (a1, b1, and b2 sites) were required by GlnR for its specific binding to the nas promoter region and efficient activation of the transcription of the nas operon in U32, while the a2 site seemed unnecessary.
All Author(s) ListWang Y, Wang JZ, Shao ZH, Yuan H, Lu YH, Jiang WH, Zhao GP, Wang J
Journal nameJournal of Bacteriology
Detailed descriptionTo ORKTS: URL fm P133617 record.
Year2013
Month6
Day1
Volume Number195
Issue Number11
PublisherAMER SOC MICROBIOLOGY
Pages2595 - 2602
ISSN0021-9193
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesMicrobiology; MICROBIOLOGY

Last updated on 2020-17-10 at 01:23