Three of Four GlnR Binding Sites Are Essential for GlnR-Mediated Activation of Transcription of the Amycolatopsis mediterranei nas Operon
Publication in refereed journal

香港中文大學研究人員

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摘要In Amycolatopsis mediterranei U32, genes responsible for nitrate assimilation formed one operon, nasACKBDEF, whose transcription is induced by the addition of nitrate. Here, we characterized GlnR as a direct transcriptional activator for the nas operon. The GlnR-protected DNA sequences in the promoter region of the nas operon were characterized by DNase I footprinting assay, the previously deduced Streptomyces coelicolor double 22-bp GlnR binding consensus sequences comprising a1, b1, a2, and b2 sites were identified, and the sites were then mutated individually to test their roles in both the binding of GlnR in vitro and the GlnR-mediated transcriptional activation in vivo. The results clearly showed that only three GlnR binding sites (a1, b1, and b2 sites) were required by GlnR for its specific binding to the nas promoter region and efficient activation of the transcription of the nas operon in U32, while the a2 site seemed unnecessary.
著者Wang Y, Wang JZ, Shao ZH, Yuan H, Lu YH, Jiang WH, Zhao GP, Wang J
期刊名稱Journal of Bacteriology
詳細描述To ORKTS: URL fm P133617 record.
出版年份2013
月份6
日期1
卷號195
期次11
出版社AMER SOC MICROBIOLOGY
頁次2595 - 2602
國際標準期刊號0021-9193
語言英式英語
Web of Science 學科類別Microbiology; MICROBIOLOGY

上次更新時間 2020-17-09 於 01:39