A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity from the Edible Mushroom Hygrophorus russula
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AbstractA 28-kDa ribonuclease, with an optimum pH of 4.0 and an optimum temperature at 58 degrees C, was isolated from fruiting bodies of the edible mushroom Hygrophorus russula. It was purified by ion exchange chromatography on carboxymethyl-cellulose, dithyaminoethyl-cellulose, quaternary amine-sepharose and sulphopropyl-sepharose, followed by fast protein liquid chromatography gel filtration on Superdex 75. The N-terminal amino acid sequence was ASAGG which showed homology to those of other fungal RNases to some degree. It exerted the highest RNase activity on poly C and poly U. The Michaelis constant (K-m) value of the RNase on yeast tRNA was 3.6 mu M, and the maximal velocity (V-max) was 0.6 mu M. The RNase activity was suppressed by some ions including Fe2+ and Zn2+ ions. The RNase inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 4.64 mu M.
All Author(s) ListZhu MJ, Xu LJ, Chen X, Ma ZQ, Wang HX, Ng TB
Journal nameApplied Biochemistry and Biotechnology
Detailed descriptionTo ORKTS: doi: 10.1007/s12010-013-0180-8
Year2013
Month5
Day1
Volume Number170
Issue Number1
PublisherHumana Press
Pages219 - 230
ISSN0273-2289
eISSN1559-0291
LanguagesEnglish-United Kingdom
KeywordsEdible mushroom; HIV-1 reverse transcriptase; Hygrophorus russula; Purification; Ribonuclease
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Last updated on 2020-31-05 at 01:31