Abnormal Skeletal Growth in Adolescent Idiopathic Scoliosis Is Associated with Abnormal Quantitative Expression of Melatonin Receptor, MT2
Publication in refereed journal


摘要The defect of the melatonin signaling pathway has been proposed to be one of the key etiopathogenic factors in adolescent idiopathic scoliosis (AIS). A previous report showed that melatonin receptor, MT2, was undetectable in some AIS girls. The present study aimed to investigate whether the abnormal MT2 expression in AIS is quantitative or qualitative. Cultured osteoblasts were obtained from 41 AIS girls and nine normal controls. Semi-quantification of protein expression by Western blot and mRNA expression by TaqMan real-time PCR for both MT1 and MT2 were performed. Anthropometric parameters were also compared and correlated with the protein expression and mRNA expression of the receptors. The results showed significantly lower protein and mRNA expression of MT2 in AIS girls compared with that in normal controls (p = 0.02 and p = 0.019, respectively). No differences were found in the expression of MT1. When dichotomizing the AIS girls according to their MT2 expression, the group with low expression was found to have a significantly longer arm span (p = 0.036). The results of this study showed for the first time a quantitative change of MT2 in AIS that was also correlated with abnormal arm span as part of abnormal systemic skeletal growth.
著者Yim APY, Yeung HY, Sun GQ, Lee KM, Ng TB, Lam TP, Ng BKW, Qiu Y, Moreau A, Cheng JCY
期刊名稱International Journal of Molecular Sciences
詳細描述To ORKTS: doi: 10.3390/ijms14036345

Clarified by Isabel Chan, Yong Qiu is a non-CU staff, though the affiliation is stated as joint lab with our Sc
頁次6345 - 6358
關鍵詞idiopathic scoliosis; melatonin; osteoblasts; receptors
Web of Science 學科類別Biochemistry & Molecular Biology; Chemistry; Chemistry, Multidisciplinary; CHEMISTRY, MULTIDISCIPLINARY

上次更新時間 2021-08-09 於 23:52