Double restriction-enzyme digestion improves the coverage and accuracy of genome-wide CpG methylation profiling by reduced representation bisulfite sequencing
Publication in refereed journal

Times Cited
Web of Science46WOS source URL (as at 19/11/2020) Click here for the latest count
Altmetrics Information

Other information
AbstractBackground: Reduced representation bisulfite sequencing (RRBS) was developed to measure DNA methylation of high-CG regions at single base-pair resolution, and has been widely used because of its minimal DNA requirements and cost efficacy; however, the CpG coverage of genomic regions is restricted and important regions with low-CG will be ignored in DNA methylation profiling. This method could be improved to generate a more comprehensive representation.
All Author(s) ListWang JW, Xia YD, Li LL, Gong DS, Yao Y, Luo HJ, Lu HL, Yi N, Wu HL, Zhang XQ, Tao Q, Gao F
Journal nameBMC Genomics
Volume Number14
LanguagesEnglish-United Kingdom
KeywordsCpG coverage; DNA methylation; Double-enzyme RRBS; Single-enzyme RRBS
Web of Science Subject CategoriesBiotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Genetics & Heredity; GENETICS & HEREDITY

Last updated on 2020-20-11 at 00:49