Cajaninstilbene Acid Relaxes Rat Renal Arteries: Roles of Ca2+ Antagonism and Protein Kinase C-Dependent Mechanism
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AbstractCajaninstilbene acid (CSA) is a major active component present in the leaves of Cajanus cajan (L.) Millsp. The present study explores the underlying cellular mechanisms for CSA-induced relaxation in rat renal arteries. Vascular reactivity was examined in arterial rings that were suspended in a Multi Myograph System and the expression of signaling proteins was assessed by Western blotting method. CSA (0.1-10 mu M) produced relaxations in rings pre-contracted by phenylephrine, serotonin, 9, 11-dideoxy-9 alpha, 11 alpha-epoxymethanoprostaglandin F-2 alpha (U46619), and 60 mM KCl. CSA-induced relaxations did not show difference between genders and were unaffected by endothelium denudation, nor by treatment with N-G-nitro-L-arginine methyl ester, indomethacin, ICI-182780, tetraethylammonium ion, BaCl2, glibenclamide, 4-aminopyridine or propranolol. CSA reduced contraction induced by CaCl2 (0.01-5 mM) in Ca2+-free 60 mM KCl solution and by 30 nM (-)-Bay K8644 in 15 mM KCl solution. CSA inhibited 60 mM KCl-induced Ca2+ influx in smooth muscle of renal arteries. In addition, CSA inhibited contraction evoked by phorbol 12-myristate 13-acetate (PMA, protein kinase C agonist) in Ca2+-free Krebs solution. Moreover, CSA reduced the U46619- and PMA-induced phosphorylation of myosin light chain (MLC) at Ser19 and myosin phosphatase target subunit 1 (MYPT1) at Thr853 which was associated with vasoconstriction. CSA also lowered the phosphorylation of protein kinase C (PKC delta) at Thr505. In summary, the present results suggest that CSA relaxes renal arteries in vitro via multiple cellular mechanisms involving partial inhibition of calcium entry via nifedipine-sensitive calcium channels, protein kinase C and Rho kinase.
All Author(s) ListZhang DM, Li Y, Cheang WS, Lau CW, Lin SM, Zhang QL, Yao N, Wang Y, Wu X, Huang Y, Ye WC
Journal namePLoS ONE
Detailed descriptionTo ORKTS: Prof. Yu Huang is the Co-corresponding Author; doi: 10.1371/journal.pone.0047030.
Deleted duplicated record (P124504) on 5-Dec-2013.
Year2012
Month10
Day9
Volume Number7
Issue Number10
PublisherPUBLIC LIBRARY SCIENCE
ISSN1932-6203
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesMultidisciplinary Sciences; MULTIDISCIPLINARY SCIENCES; Science & Technology - Other Topics

Last updated on 2020-02-04 at 01:08