Bisphenol A induces corticotropin-releasing hormone expression in the placental cells JEG-3
Publication in refereed journal


摘要Bisphenol A is utilized to make polycarbonate plastics and is an environmental pollutant. Recent research has indicated that it is an endocrine disruptor and may interfere with reproduction. Placental corticotrophin-releasing hormone (CRH) is a peptide hormone which is involved in fetal development. Increased plasma CRH is associated with elevated risk of premature delivery. In the present study, we demonstrated that bisphenol A increased CRH mRNA expression in the placental JEG-3 cells at or above 25 mu M. Reporter gene assay also demonstrated that bisphenol A could induce CRH gene transactivity. Since cyclic AMP response element (CRE) is a major regulatory element located in CRH promoter, the sequence-specific binding activity was investigated by using electrophoretic mobility shift assay. Our data indicated that bisphenol A increased the CRE binding activity. Western analysis further illustrated that PKA could be the signal triggering the CRE binding and CRH gene transactivation. In summary, the present study demonstrated that bisphenol A could induce CRH expression in placental cells and the underlying signal transduction pathway was also described. (c) 2012 Elsevier Inc. All rights reserved.
著者Huang H, Tan WJ, Wang CC, Leung LK
期刊名稱Reproductive Toxicology
頁次317 - 322
關鍵詞Bisphenol A; CRH; Placental cells
Web of Science 學科類別Reproductive Biology; REPRODUCTIVE BIOLOGY; Toxicology; TOXICOLOGY

上次更新時間 2021-29-11 於 00:13