Uniaxial mechanical tension promoted osteogenic differentiation of rat tendon-derived stem cells (rTDSCs) via the Wnt5a-RhoA pathway
Publication in refereed journal

香港中文大學研究人員
作者已離職


引用次數
替代計量分析
.

其它資訊
摘要Chronic tendinopathy is a tendon disorder that is common in athletes and individuals whose tendons are subjected to repetitive strain injuries. The presence of ossification worsened the clinical manifestation of the disorder. The change of tendon loading due to mechanical overload, compression, or disuse have been implicated as the possible etiologies, but the pathological mechanisms of tendinopathy remain unclear. In this study, we demonstrated that ossification in tendon tissue might be due to the osteogenesis of tendon-derived stem cells (TDSCs) induced by uniaxial mechanical tension (UMT) which mimics the mechanical loading in tendon. Rat TDSCs (rTDSCs) could be induced to differentiate into osteogenic lineage after treatment with 2% elongation UMT for 3 days as shown by the increased expression Runx2 mRNA and protein, Alpl mRNA, collagen type 1 alpha 1 (Col1a1) mRNA, ALP activity, and ALP cytochemical staining. RhoA, an osteogenesis regulator, was activated in rTDSCs upon UMT stimulation. Blockage of RhoA activity in rTDSCs by C3 toxin or ROCK activity, a downstream target of RhoA, by Y-27632 inhibited UMT-induced osteogenesis in rTDSCs. UMT up-regulated the mRNA expression of Wnt5a but not the other non-canonical Wnts. The inhibition of Wnt5a expression by siRNA abolished UMT-induced Runx2 mRNA expression and RhoA activation in rTDSCs and the inhibition of Runx2 expression could be rescued by addition of LPA, a RhoA activator. In conclusion, our results showed that UMT induced osteogenic differentiation of rTDSCs via the Wnt5a-RhoA pathway, which might contribute to ectopic ossification in tendon tissue due to mechanical loading. J. Cell. Biochem. 113: 31333142, 2012.(c) 2012 Wiley Periodicals, Inc.
著者Shi Y, Fu YJ, Tong WX, Geng YY, Lui PPY, Tang TT, Zhang XL, Dai KR
期刊名稱Journal of Cellular Biochemistry
出版年份2012
月份10
日期1
卷號113
期次10
出版社Wiley: 12 months
頁次3133 - 3142
國際標準期刊號0730-2312
電子國際標準期刊號1097-4644
語言英式英語
關鍵詞OSTEOGENIC DIFFERENTIATION; RAT TENDON DERIVED STEM CELLS (rTDSCs); RhoA; Runx2; UNIAXIAL MECHANICAL TENSION (UMT); Wnt5A
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY

上次更新時間 2020-18-10 於 00:42