PEG-nanolized ultrasmall selenium nanoparticles overcome drug resistance in hepatocellular carcinoma HepG2 cells through induction of mitochondria dysfunction
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摘要Gray selenium (Se) is one of the most widely used Se sources with very limited biocompatibility and bioactivity. In the present study, a simple method for the preparation of ultrasmall selenium nanoparticles (SeNPs) through direct nanolization of gray selenium by polyethylene glycol (PEG) was demonstrated. Monodisperse and homogeneous PEG-SeNPs with ultrasmall diameters were successfully prepared under optimized conditions. The products were characterized using various microscopic and spectroscopic methods, and the results suggest that the amphoteric properties of PEG and the coordination between oxygen and selenium atoms contributed to the formation of ultrasmall nanoparticles. PEG-SeNPs exhibited stronger growth inhibition on drug-resistant hepatocellular carcinoma (R-HepG2) cells than on normal HepG2 cells. Dose-dependent apoptosis was induced by PEG-SeNPs in R-HepG2 cells, as evidenced by an increase in the sub-G1 cell population. Further investigation on the underlying molecular mechanisms revealed that depletion of mitochondrial membrane potential and generation of superoxide anions contributed to PEG-SeNPs-induced apoptotic cell death in R-HepG2 cells. Our results suggest that PEG-SeNPs may be a candidate for further evaluation as a chemotherapeutic agent for drug-resistant liver cancer, and the strategy to use PEG200 as a surface decorator could be a highly efficient way to enhance the anticancer efficacy of nanomaterials.
著者Zheng SY, Li XL, Zhang YB, Xie Q, Wong YS, Zheng WJ, Chen TF
期刊名稱International Journal of Nanomedicine
出版年份2012
月份1
日期1
卷號7
出版社DOVE MEDICAL PRESS LTD
頁次3939 - 3949
國際標準期刊號1176-9114
語言英式英語
關鍵詞apoptosis; drug resistance; nanolization; PEG; selenium
Web of Science 學科類別Nanoscience & Nanotechnology; NANOSCIENCE & NANOTECHNOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Science & Technology - Other Topics

上次更新時間 2021-22-01 於 23:57