Activation of the epithelial Na+ channel triggers prostaglandin E-2 release and production required for embryo implantation
Publication in refereed journal


摘要Embryo implantation remains a poorly understood process. We demonstrate here that activation of the epithelial Na+ channel (ENaC) in mouse endometrial epithelial cells by an embryo-released serine protease, trypsin, triggers Ca2+ influx that leads to prostaglandin E-2 (PGE(2)) release, phosphorylation of the transcription factor CREB and upregulation of cyclooxygenase 2, the enzyme required for prostaglandin production and implantation(1-3). We detected maximum ENaC activation, as indicated by ENaC cleavage(4), at the time of implantation in mice. Blocking or knocking down uterine ENaC in mice resulted in implantation failure. Furthermore, we found that uterine ENaC expression before in vitro fertilization (IVF) treatment is markedly lower in women with implantation failure as compared to those with successful pregnancy. These results indicate a previously undefined role of ENaC in regulating the PGE(2) production and release required for embryo implantation, defects that may be a cause of miscarriage and low success rates in IVF.
著者Ruan YC, Guo JH, Liu XM, Zhang RJ, Tsang LL, Dong JD, Chen H, Yu MK, Jiang XH, Zhang XH, Fok KL, Chung YW, Huang HF, Zhou WL, Chan HC
期刊名稱Nature Medicine
詳細描述To ORKTS: doi: 10.1038/nm.2771
出版社Nature Publishing Group
頁次1112 - +
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine

上次更新時間 2021-23-02 於 23:56