Activation of the epithelial Na+ channel triggers prostaglandin E-2 release and production required for embryo implantation
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AbstractEmbryo implantation remains a poorly understood process. We demonstrate here that activation of the epithelial Na+ channel (ENaC) in mouse endometrial epithelial cells by an embryo-released serine protease, trypsin, triggers Ca2+ influx that leads to prostaglandin E-2 (PGE(2)) release, phosphorylation of the transcription factor CREB and upregulation of cyclooxygenase 2, the enzyme required for prostaglandin production and implantation(1-3). We detected maximum ENaC activation, as indicated by ENaC cleavage(4), at the time of implantation in mice. Blocking or knocking down uterine ENaC in mice resulted in implantation failure. Furthermore, we found that uterine ENaC expression before in vitro fertilization (IVF) treatment is markedly lower in women with implantation failure as compared to those with successful pregnancy. These results indicate a previously undefined role of ENaC in regulating the PGE(2) production and release required for embryo implantation, defects that may be a cause of miscarriage and low success rates in IVF.
All Author(s) ListRuan YC, Guo JH, Liu XM, Zhang RJ, Tsang LL, Dong JD, Chen H, Yu MK, Jiang XH, Zhang XH, Fok KL, Chung YW, Huang HF, Zhou WL, Chan HC
Journal nameNature Medicine
Detailed descriptionTo ORKTS: doi: 10.1038/nm.2771
Year2012
Month7
Day1
Volume Number18
Issue Number7
PublisherNature Publishing Group
Pages1112 - +
ISSN1078-8956
eISSN1546-170X
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine

Last updated on 2020-18-10 at 00:36