Total Flavonoid Fraction of Rhizoma Drynaria Improves Bone Properties in Ovariectomized Mice and Exerts Estrogen-Like Activities in Rat Osteoblast-Like (UMR-106) Cells
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AbstractRhizoma Drynaria (RD) has been widely used for healing bone fractures or related diseases in traditional Chinese medicine. The present study was designed to determine if RD total flavonoids (RDTF) could exert estrogen-like protective actions on bone. Young C57/BL6J mice were ovariectomized (OVX) and treated orally with RDTF (0.087, 0.173 or 0.346 mg/g/day), 17 beta-estradiol (2 mu g/g/day) or its vehicle for 6 weeks. Bone mineral densities (BMD) was measured by peripheral quantitative computed tomography (pQCT). Rat osteoblast-like UMR-106 cells were co-incubated with ER antagonist ICI 182, 780 to determine if the effects of RDTF on osteoblastic functions were ER-dependent. The functional transactivation of ER-alpha and ER-beta as well as ER-alpha phosphorylation by RDTF were also studied. RDTF increased trabecular-rich BM D at distal femur and lumbar spine in OVX mice. 0.173 mg/g/day was most effective in improving bone properties in OVX mice. The stimulatory effects of RDTF on osteoblastic functions could be abolished by co-incubation with ICI 182, 780 in UMR-106 cells. Transient transfection study indicated that RDTF dose-dependently stimulated ERE-dependent luciferase activity in UMR-106 cells via ER-alpha and ER-beta. Moreover, 0.2 mu g/mL of RDTF significantly induced ER-alpha phosphorylation at serine 118 in UMR-106 cells.
All Author(s) ListPang WY, Wang XL, Wong KC, Leung PC, Yao XS, Wong MS
Name of Conference5th International Conference on Food Factors (ICoFF)
Start Date of Conference20/11/2011
End Date of Conference23/11/2011
Place of ConferenceTaipei
Journal nameJournal of Food and Drug Analysis
Year2012
Month4
Day1
Volume Number20
PublisherBUREAU FOOD DRUG ANALYSIS
Pages265 - 269
ISSN1021-9498
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesFood Science & Technology; FOOD SCIENCE & TECHNOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-28-10 at 00:49