A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus
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AbstractA 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC50 = 2.4 mu M) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion exchangers. About 95-fold purification was achieved with a 25.9% yield of the enzyme. The procedure resulted in a specific enzyme activity of 76.6 U/mg. Its N-terminal amino acid sequence was GIPDLHDLTV, which showed little similarity to other mushroom laccase and other Lentinus tigrinus strain laccase. Its characteristics were different from previously reported laccase of other Lentinus tigrinus strain. Maximal laccase activity was observed at a pH of 4 and at a temperature of 60 degrees C, respectively. This study yielded the information about the potentially exploitable activities of Lentinus tigrinus laccase.
All Author(s) ListXu LJ, Wang HX, Ng T
Journal nameJournal of Biomedicine and Biotechnology
Detailed descriptionTo ORKTS: doi: 10.1155/2012/536725
Year2012
Month1
Day1
PublisherHINDAWI PUBLISHING CORPORATION
ISSN1110-7243
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesBiotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine

Last updated on 2020-24-11 at 00:31