Triptolide Down-regulates COX-2 Expression and PGE2 Release by Suppressing the Activity of NF-?B and MAP kinases in Lipopolysaccharide-treated PC12 Cells
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AbstractAs an active compound extracted from the Chinese herb Tripterygium wilfordii, triptolide (TP) was demonstrated to have potent antiinflammatory and immunosuppressive properties in previous studies. Recently, it has been shown that TP prevented the loss of dopaminergic neurons in the substantia nigra of rats in a model of Parkinson's disease, but little is known about the precise neuroprotective mechanism of TP. This study was designed to elucidate whether the neuroprotective effect of TP is partially based on its direct inhibition of inflammatory molecules by investigating the effects of TP on the expression of cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2) related to the nuclear factor (NF)-?B pathway in lipopolysaccharide (LPS)-stimulated PC12 cells. The activation of related upstream molecules such as NF-?B, P38, extracellular signal-regulated kinase (ERK)1/2, and beta-alanyl-alpha-ketoglutarate transaminase (AKT), in PC12 cells were investigated by real time polymerase chain reaction (PCR), western blotting and enzyme-linked immunosorbent assay (ELISA). Our results showed that TP directly inhibited the expression of both mRNA and protein of COX-2 (p?
All Author(s) ListGeng Y, Fang MR, Wang J, Yu HY, Hu ZY, Yew DT, Chen W
Journal namePhytotherapy Research
Detailed descriptionTo ORKTS: DOI: 10.1002/ptr.3538
Year2012
Month3
Day1
Volume Number26
Issue Number3
PublisherWiley: 12 months
Pages337 - 343
ISSN0951-418X
eISSN1099-1573
LanguagesEnglish-United Kingdom
Keywordscyclooxgenase-2; lipopolysaccharide; neurodegenerative; nuclear factor-kappa B; PC12 cells; triptolide
Web of Science Subject CategoriesChemistry, Medicinal; CHEMISTRY, MEDICINAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-13-10 at 00:49