Biodegradation of dyes and polyaromatic hydrocarbons by two allelic forms of Lentinula edodes laccase expressed from Pichia pastoris
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摘要Laccases from basidiomycetes are efficient enzymes in the degradation of xenobiotics. In this study we aimed to provide an industrially relevant expression system for Lentinula edodes laccases, to characterize their enzymatic properties, and to evaluate their potential in bioremediation. Two 1573-bp allelic laccase genes from L. edodes L54 were cloned based on gene models in the genome sequence. A novel upstream consensus (GCTCCGA/CCGGAG) was proposed as an alternative signature sequence for laccases. Both alleles were overexpressed in Pichia pastoris, purified, and verified by zymograms. Kinetic analyses suggested an order of catalytic efficiency of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) > 2,6-dimethoxyphenol > guaiacol > L-3,4-dihydroxyphenylalanine > catechol, and a stable range of working temperature below 40 degrees C. With the appropriate mediators, 1-hydroxybenzotriazole and 2,2,6,6-tetra-methylpiperidine-1-oxyl, the recombinant enzymes could catalyze a 70-100% decolorization of selected dyes and a complete degradation of anthracene. These results laid a solid foundation for the use of L edodes laccases in bioremediations and for improvement with protein engineering. (C) 2011 Elsevier Ltd. All rights reserved.
著者Wong KS, Huang QL, Au CH, Wang J, Kwan HS
期刊名稱Bioresource Technology
出版年份2012
月份1
日期1
卷號104
出版社Elsevier
頁次157 - 164
國際標準期刊號0960-8524
電子國際標準期刊號1873-2976
語言英式英語
關鍵詞Dye; Laccase; Lentinula edodes; Pichia pastoris; Polyaromatic hydrocarbon
Web of Science 學科類別Agricultural Engineering; AGRICULTURAL ENGINEERING; Agriculture; Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Energy & Fuels; ENERGY & FUELS

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