Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo
Publication in refereed journal



摘要Astragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (H U V ECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expressionvascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase-domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1 /VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both V EGF- and Akt-dependent signaling pathways.
著者Zhang Y, Hu G, Li S, Li ZH, Lam CO, Hong SJ, Kwan YW, Chan SW, Leung GPH, Lee SMY
期刊名稱Molecular Medicine Reports
詳細描述To ORKTS: doi: 10.3892/mmr.2011.716
出版社Spandidos Publications
頁次805 - 811
關鍵詞angiogenesis; astragaloside IV; human umbilical vein endothelial cell; Radix Astragai; zebrafish
Web of Science 學科類別Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Oncology; ONCOLOGY; Research & Experimental Medicine

上次更新時間 2021-12-01 於 23:42