Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo
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AbstractAstragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (H U V ECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expressionvascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase-domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1 /VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both V EGF- and Akt-dependent signaling pathways.
All Author(s) ListZhang Y, Hu G, Li S, Li ZH, Lam CO, Hong SJ, Kwan YW, Chan SW, Leung GPH, Lee SMY
Journal nameMolecular Medicine Reports
Detailed descriptionTo ORKTS: doi: 10.3892/mmr.2011.716
Year2012
Month3
Day1
Volume Number5
Issue Number3
PublisherSpandidos Publications
Pages805 - 811
ISSN1791-2997
eISSN1791-3004
LanguagesEnglish-United Kingdom
Keywordsangiogenesis; astragaloside IV; human umbilical vein endothelial cell; Radix Astragai; zebrafish
Web of Science Subject CategoriesMedicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Oncology; ONCOLOGY; Research & Experimental Medicine

Last updated on 2020-17-11 at 00:47